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KMID : 1007520100190061567
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Food Science and Biotechnology
2010 Volume.19 No. 6 p.1567 ~ p.1571
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Optimization of Medium on Exopolysaccharides Production in Submerged Culture of Cordyceps militaris
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Cui Jian-Dong
Jia Shi-Ru
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Abstract
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Statistical experimental design strategy (SES) was applied to optimize the medium for the exopolysaccharides (EPS) production of Cordyceps militaris by submerged culture in shaker flask. A significant influence of the glucose and peptone on the EPS production was first evaluated by using a Plackett-Buman design. Then, steepest ascent method was employed to approach the experimental design space. Last, these factors were further optimized using central composite design (CCD) and response surface methodology (RSM). A quadratic model was found to fit the EPS production. The optimum values of the tested variables for the production of EPS were 48.67 g/L glucose, 12.56 g/L peptone, 1 g/L KH2PO4, 10 g/L yeast extract, and 0.5 g/L MgSO4·7H2O. Under optimization of culture conditions, the EPS production was enhanced from 0.78 to 1.96 g/L. In comparison with that of original culture conditions, 2.5 fold increase was obtained.
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KEYWORD
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medium, optimization, exopolysaccharides (EPS) production, Cordyceps militaris, statistic experimental design
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